A major advantage of monoclonal antibodies compared to polyclonal antibodies is that monoclonal antibodiesShow answer
(A) have identical binding sites that recognize a specific epitope
(B) cross-link molecules that share antigenic sites
(C) are more easily coupled with probes such as fluorescent dyes
(D) have higher-affinity binding to antigens
(E) can be produced against proteins that are immunogenic in rabbits
Here we again should define used words accurately to find right answer. Antibodies are polypeptides, consisted of four chains, that can bind to specific sites of specific molecules (antigens). Monoclonal antibodies are ones that can bind to unique site of exactly one antigen. Polyclonal antibodies bind to wide range of sites of the antigen. That sites are called antigenic determinants or epitopes. They are recognized by antibody's so-called paratope. Thus, right answer is A.
Antibodies are produced using wide range of animals (rabbits, rats etc), so E is also false answer. And all those antibodies are mainly polyclonal, but monoclonal antibodies can be extracted using special technique called postfusional selection.
Answer C is not good one, because third tail of antibodies (they are shaped as Y) is not depend on poly- or monoclonality.
Answer D is sure false, because polyclonal antibodies have higher affinity, obviously: they can hook molecules by different sites so probability of binding act is higher. B is false too, bacause of mentioned above: cross-links are formed with polyclonal antibodies more rapidly.
Antibodies are produced using wide range of animals (rabbits, rats etc), so E is also false answer. And all those antibodies are mainly polyclonal, but monoclonal antibodies can be extracted using special technique called postfusional selection.
Answer C is not good one, because third tail of antibodies (they are shaped as Y) is not depend on poly- or monoclonality.
Answer D is sure false, because polyclonal antibodies have higher affinity, obviously: they can hook molecules by different sites so probability of binding act is higher. B is false too, bacause of mentioned above: cross-links are formed with polyclonal antibodies more rapidly.
